7R1Z
C-terminal domain of hArc in complex with nanobodies H11 and C11, collapsed crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.260, 139.290, 43.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 1.940 |
R-factor | 0.2005 |
Rwork | 0.197 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6tnp |
RMSD bond length | 0.003 |
RMSD bond angle | 0.608 |
Data reduction software | XDS (Feb 5, 2021) |
Data scaling software | XSCALE (Feb 5, 2021) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (dev_3958) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.990 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmeas | 0.130 | 2.356 |
Number of reflections | 26403 | 1025 |
<I/σ(I)> | 13.13 | 0.83 |
Completeness [%] | 0.9 | 46.2 |
Redundancy | 11.9 | 6.72 |
CC(1/2) | 0.990 | 0.351 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 17% PEG3350. Produced from seed crystals grown in 12% PEG3350. |