6TNP

Crystal structure of the ScFv-5E5 in complex with a Tn glycopeptide

Summary for 6TNP

• Entry DOI: 10.2210/pdb6tnp/pdb
• Descriptor: Light chain of our ScFv-5E5, Heavy chain of our ScFv-5E5, (2~{S})-~{N}-[2-[[(2~{S})-1-[[(2~{S},3~{R})-3-[(2~{S},3~{R},4~{R},5~{R},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]oxy-1-[[(2~{S})-1-[(2~{S})-2-aminocarbonylpyrrolidin-1-yl]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]amino]-2-oxidanylidene-ethyl]-1-[(2~{S})-2-azanylpropanoyl]pyrrolidine-2-carboxamide, ... (4 entities in total)
• Functional Keywords: antibody, scfv, 5e5, anti-tn antibody, muc1, cancer, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 12
• Total formula weight: 156140.52

Authors

Macias-Leon, J.,Corzana, F.,Hurtado-Guerrero, R. (deposition date: 2019-12-09, release date: 2020-11-18, Last modification date: 2024-11-13)

Primary citation

Macias-Leon, J.,Bermejo, I.A.,Asin, A.,Garcia-Garcia, A.,Companon, I.,Jimenez-Moreno, E.,Coelho, H.,Mangini, V.,Albuquerque, I.S.,Marcelo, F.,Asensio, J.L.,Bernardes, G.J.L.,Joshi, H.J.,Fiammengo, R.,Blixt, O.,Hurtado-Guerrero, R.,Corzana, F.
Structural characterization of an unprecedented lectin-like antitumoral anti-MUC1 antibody.
Chem.Commun.(Camb.), 56:15137-15140, 2020

PubMed Abstract: The molecular basis of antibody 5E5, which recognizes the entire GalNAc unit as a primary epitope is disclosed. The antibody's contacts with the peptide are mostly limited to two residues, allowing it to show some degree of promiscuity. These findings open the door to the chemical design of peptide-mimetics for developing efficient anti-cancer vaccines and diagnostic tools.

PubMed: 33211039
DOI: 10.1039/d0cc06349e

Experimental method

X-RAY DIFFRACTION (3 Å)