7R1C
Cryo-EM structure of Bacillus megaterium gas vesicles
EM specimen
Vitrification | Yes |
Instrument | LEICA PLUNGER |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.2 |
Number of Particle | 36295 |
Software | RELION,cryoSPARC,PHENIX,ISOLDE |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |