7QZQ

Crystal structure of the kelch domain of human KBTBD12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	72.379, 55.078, 95.164
Unit cell angles	90.00, 108.67, 90.00

Refinement procedure

Resolution	45.080 - 1.880
R-factor	0.1971
Rwork	0.195
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vpj
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.630	1.920
High resolution limit [Å]	1.880	1.880
Rmerge	0.156	3.100
Rmeas	0.170
Number of reflections	57704	2827
<I/σ(I)>	7.3	0.5
Completeness [%]	99.9	97.7
Redundancy	6.4	5.6
CC(1/2)	0.997	0.268

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293.15	8% PEG4000 -- 0.1M acetate pH 4.5