7QZ1
Formate dehydrogenase from Starkeya novella
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99 |
Spacegroup name | P 1 |
Unit cell lengths | 59.255, 59.481, 122.673 |
Unit cell angles | 79.89, 85.41, 63.15 |
Refinement procedure
Resolution | 52.870 - 2.100 |
R-factor | 0.178 |
Rwork | 0.176 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nac |
RMSD bond length | 0.007 |
RMSD bond angle | 0.884 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.870 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 79594 | 7804 |
<I/σ(I)> | 9.89 | |
Completeness [%] | 91.5 | |
Redundancy | 3.8 | |
CC(1/2) | 0.997 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 5-20% PEG 3350, 0.1 M BIS-TRIS pH 5.8-7.2 |