7QU1

Machupo virus GP1 glycoprotein in complex with Fab fragment of antibody MAC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	91.692, 100.731, 164.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.370 - 1.910
R-factor	0.1962
Rwork	0.194
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nuz
RMSD bond length	0.006
RMSD bond angle	0.818
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.370	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.118	1.515
Number of reflections	59374	2930
<I/σ(I)>	7.3
Completeness [%]	100.0	99.4
Redundancy	6.8	6.6
CC(1/2)	0.998	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1.4 M sodium malonate (pH 6.0)