7QTX

Kaposi sarcoma associated herpes virus (KSHV) encoded apoptosis inhibitor, KsBcl-2 in complex with Puma BH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.937
Spacegroup name	C 1 2 1
Unit cell lengths	59.229, 48.529, 57.386
Unit cell angles	90.00, 97.06, 90.00

Refinement procedure

Resolution	32.463 - 2.116
R-factor	0.219570195451
Rwork	0.218
R-free	0.25749
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	KsBcl-2_Bid
RMSD bond length	0.002
RMSD bond angle	0.435
Data reduction software	DIALS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.463	2.170
High resolution limit [Å]	2.110	2.110
Number of reflections	9148	409
<I/σ(I)>	4.2
Completeness [%]	98.1
Redundancy	2.4
CC(1/2)	0.980	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium bromide, 0.1 M Bis-tris propane pH 6.5, 20% PEG 3350