7QLA
Structure of the Rab GEF complex Mon1-Ccz1
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.001 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.85 |
| Number of Particle | 7155250 |
| Software | crYOLO,RELION,Coot,EPU,MotionCorr2,SPHIRE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
