7QH8
CRYSTAL STRUCTURE OF LYSYL-TRNA SYNTHETASE FROM Mycobacterium tuberculosis COMPLEXED WITH L-LYSINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.838, 83.838, 147.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.350 - 1.920 |
| R-factor | 0.2129 |
| Rwork | 0.211 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5elo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.543 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.890 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.095 | 3.304 |
| Rmeas | 0.099 | |
| Rpim | 0.026 | 0.992 |
| Number of reflections | 41053 | 2007 |
| <I/σ(I)> | 15.1 | 1 |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 13.8 | 12 |
| CC(1/2) | 0.999 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Reservoir: 0.25 M NaOAc, 14% w/v PEG 3350 Protein buffer: 25 mM HEPES, 500 mM NaCl, 5% glycerol, 2 mM DTT, pH 7.0 |
