7QGI
Crystal structure of SARS-CoV-2 NSP14 in the absence of NSP10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9179 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.539, 68.093, 138.428 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.100 - 1.650 |
R-factor | 0.1968 |
Rwork | 0.196 |
R-free | 0.22070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5c8s |
RMSD bond length | 0.002 |
RMSD bond angle | 0.507 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.214 | 1.850 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.125 | 1.075 |
Rpim | 0.059 | 0.466 |
Number of reflections | 37654 | 1884 |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 89.2 | 65.6 |
Redundancy | 5.5 | 6.2 |
CC(1/2) | 0.997 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1.26 M sodium phosphate monobasic, 0.14 M potassium phosphate dibasic |