7QF0

Crystal structure of the SARS-CoV-2 RBD in complex with the human antibody CV2.2325

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2020-06-07
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9778
Spacegroup name	C 2 2 21
Unit cell lengths	84.565, 148.875, 145.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.800 - 2.300
R-factor	0.1867
Rwork	0.185
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 5i1e
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.360	48.360	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.147	0.040	1.188
Rmeas	0.153	0.042	1.236
Rpim	0.041	0.012	0.337
Total number of observations	560786	9359	52704
Number of reflections	40972	792	3971
<I/σ(I)>	17.4	47.8	2.4
Completeness [%]	99.9	99.3	99.7
Redundancy	13.7	11.8	13.3
CC(1/2)	0.998	0.999	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	296	0.1 M ammonium citrate (pH 7.0), 12% PEG 3350,