7QEZ

Crystal structure of the SARS-CoV-2 RBD in complex with the ultrapotent antibody CV2.1169 and CR3022

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 41
Unit cell lengths	97.751, 97.751, 164.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.880 - 2.890
R-factor	0.2409
Rwork	0.239
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j 7k3q 6yla
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.880	48.880	3.020
High resolution limit [Å]	2.880	9.560	2.880
Rmerge	0.261	0.062	3.509
Rmeas	0.271	0.064	3.643
Rpim	0.072	0.018	0.971
Total number of observations	485839	12934	61055
Number of reflections	34600	986	4402
<I/σ(I)>	10.2	36.8	0.9
Completeness [%]	99.4	99.2	96
Redundancy	14	13.1	13.9
CC(1/2)	0.995	0.996	0.214

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	15% PEG 8000, 0.5 M lithium sulfate