7QEM
bacterial IMPDH chimera
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 4 |
Unit cell lengths | 143.320, 143.320, 119.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.770 - 3.090 |
R-factor | 0.2029 |
Rwork | 0.199 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dqw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.296 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.770 | 3.150 |
High resolution limit [Å] | 3.070 | 3.070 |
Rmerge | 0.415 | 2.788 |
Rmeas | 0.432 | 2.914 |
Rpim | 0.119 | 0.832 |
Number of reflections | 44463 | 2450 |
<I/σ(I)> | 6 | 1 |
Completeness [%] | 98.0 | 73.4 |
Redundancy | 13.1 | 11.2 |
CC(1/2) | 0.981 | 0.314 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1%w/v Tryptone, 0.05 M HEPES 7 pH, 12%w/v PEG 3350, 0.001% w/V NaN3 |