7QBE
TC:CD320 in complex with nanobody TC-Nb11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.717, 195.831, 198.385 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.960 - 3.000 |
R-factor | 0.2539 |
Rwork | 0.252 |
R-free | 0.28370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zrp |
RMSD bond length | 0.013 |
RMSD bond angle | 2.209 |
Data reduction software | X-Area |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.970 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 81479 | 8030 |
<I/σ(I)> | 11.45 | |
Completeness [%] | 99.8 | |
Redundancy | 2 | |
CC(1/2) | 0.997 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.2 M sodium malonate pH 6.0, 20% w/v PEG 3350 |