Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-11 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.368, 60.664, 118.254 |
| Unit cell angles | 90.00, 102.93, 90.00 |
Refinement procedure
| Resolution | 45.982 - 2.200 |
| Rwork | 0.199 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | internal modle |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.679 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.050 | 0.670 |
| Number of reflections | 34961 | 4068 |
| <I/σ(I)> | 16.2 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mM Tris pH 8.0, 5mM DTT, 100mM NaCl , 2mM EDTA |
