7QAR
Serial crystallography structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-methyl uric acid at room temperature
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-04-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.790, 96.030, 105.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.070 - 2.300 |
Rwork | 0.218 |
R-free | 0.26610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cw2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.388 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.070 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 18280 | 1796 |
<I/σ(I)> | 2.44 | 0.52 |
Completeness [%] | 100.0 | 100 |
Redundancy | 28.2 | 20.6 |
CC(1/2) | 0.849 | 0.225 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.6 | 291 | TRIS-ACETATE, PEG 8000, MUA, |