7QAR
Serial crystallography structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-methyl uric acid at room temperature
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.790, 96.030, 105.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.070 - 2.300 |
| Rwork | 0.218 |
| R-free | 0.26610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cw2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.388 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.070 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 18280 | 1796 |
| <I/σ(I)> | 2.44 | 0.52 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 28.2 | 20.6 |
| CC(1/2) | 0.849 | 0.225 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.6 | 291 | TRIS-ACETATE, PEG 8000, MUA, |
