7Q6K

Crystal structure of the human GDAP1 CMT2 mutant-R120W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-11-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 63 2 2
Unit cell lengths	147.270, 147.270, 114.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.210 - 3.410
R-factor	0.2545
Rwork	0.253
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7alm
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.210	48.210	3.500
High resolution limit [Å]	3.410	15.250	3.410
Rmerge	0.388	0.060	3.977
Rmeas	0.404	0.064	4.147
Total number of observations	134163
Number of reflections	10468	150	759
<I/σ(I)>	6.54	26.43	0.81
Completeness [%]	99.9	94.3	99.7
Redundancy	12.816	9.933	12.503
CC(1/2)	0.995	0.996	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	HEPES, PEG 6000