7Q6C

complement C6 FIM1-2 bound to CP010 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	161.057, 63.527, 128.589
Unit cell angles	90.00, 128.36, 90.00

Refinement procedure

Resolution	44.450 - 2.293
R-factor	0.2092
Rwork	0.208
R-free	0.23290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6and
RMSD bond length	0.005
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.826	2.430
High resolution limit [Å]	2.293	2.293
Rmerge	0.063
Rmeas	0.075
Number of reflections	45105	6899
<I/σ(I)>	12.08
Completeness [%]	97.2
Redundancy	3.46
CC(1/2)	0.999	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	291	112 mM calcium acetate, 56 mM sodium cacodylate pH 6.4, 8.5% PEG8000 and 16% glycerol