7Q6C
complement C6 FIM1-2 bound to CP010 antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.057, 63.527, 128.589 |
Unit cell angles | 90.00, 128.36, 90.00 |
Refinement procedure
Resolution | 44.450 - 2.293 |
R-factor | 0.2092 |
Rwork | 0.208 |
R-free | 0.23290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6and |
RMSD bond length | 0.005 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.826 | 2.430 |
High resolution limit [Å] | 2.293 | 2.293 |
Rmerge | 0.063 | |
Rmeas | 0.075 | |
Number of reflections | 45105 | 6899 |
<I/σ(I)> | 12.08 | |
Completeness [%] | 97.2 | |
Redundancy | 3.46 | |
CC(1/2) | 0.999 | 0.399 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.4 | 291 | 112 mM calcium acetate, 56 mM sodium cacodylate pH 6.4, 8.5% PEG8000 and 16% glycerol |