7Q5N

Crystal structure of Chaetomium thermophilum Ahp1-Urm1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	185.430, 197.220, 139.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.470 - 2.500
R-factor	0.2362
Rwork	0.234
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dsr
RMSD bond length	0.006
RMSD bond angle	0.981
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.570
High resolution limit [Å]	2.500	2.500
Rmeas	0.102	4.236
Number of reflections	87068	8611
<I/σ(I)>	10.94
Completeness [%]	99.3
Redundancy	6.74
CC(1/2)	0.999	0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.05 M Zinc Acetate Dihydrate, 0.1 M Sodium Cacodylate pH 6.5, 4 % w/v polyethylene glycol 8000, 30 % w/v ethylene glycol