7Q45

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of Myelin transcription factor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999985
Spacegroup name	P 43 21 2
Unit cell lengths	108.390, 108.390, 242.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.473 - 2.100
R-factor	0.186274376691
Rwork	0.184
R-free	0.22437
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.004
RMSD bond angle	0.788
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.473	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	84379	6077
<I/σ(I)>	9.67
Completeness [%]	99.2
Redundancy	26.794735657
CC(1/2)	0.995	0.456

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293.15	hanging drop 20% PEG 3350 0.18M tris amonium citrate