7Q44

Crystal structure of RCC1-Like domain 2 of ubiquitin ligase HERC2 in complex with DXDKDED motif of deubiquitinase USP35

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-10
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000029
Spacegroup name	P 43 21 2
Unit cell lengths	108.460, 108.460, 242.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.516 - 2.200
R-factor	0.184693201749
Rwork	0.182
R-free	0.23105
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kci
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.516	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	74354	5417
<I/σ(I)>	8.65
Completeness [%]	99.9
Redundancy	26.7427710681
CC(1/2)	0.994	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	20% PEG 3350 0.18M tris amonium citrate