7Q1E

CPAP:TUBULIN:IIH5 ALPHAREP COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	53.590, 68.050, 420.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.840 - 2.700
R-factor	0.2025
Rwork	0.200
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gwd
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (6-FEB-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.600	2.800
High resolution limit [Å]	2.700	2.700
Rmeas	0.212	5.390
Number of reflections	43707	4416
<I/σ(I)>	12.23
Completeness [%]	100.0	100
Redundancy	35.6	36.1
CC(1/2)	0.999	0.339

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.8	293	8% PEG3350, 0.1M MES-K