7Q00
Crystal structure of serine hydroxymethyltransferase, isoform 4 from Arabidopsis thaliana (SHM4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.091, 130.914, 121.778 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 49.890 - 1.740 |
| R-factor | 0.1618 |
| Rwork | 0.162 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6smr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.840 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.077 | 0.592 |
| Number of reflections | 181530 | 29467 |
| <I/σ(I)> | 8.62 | 1.99 |
| Completeness [%] | 95.5 | |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.994 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | D9 Index (Hampton Research); 0.1 M Tris 8.5, 25% w/v, Polyethylene glycol 3,350 cryoprotection with the reservoir condition supplemented with 20% ethylene glycol |
