7PU5
Structure of SFPQ-NONO complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 466.999, 66.820, 126.893 |
Unit cell angles | 90.00, 105.89, 90.00 |
Refinement procedure
Resolution | 48.450 - 2.999 |
R-factor | 0.2246 |
Rwork | 0.223 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ifm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.860 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (16-JUL-2021)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.450 | 3.200 |
High resolution limit [Å] | 2.999 | 3.000 |
Rmerge | 0.130 | |
Rpim | 0.069 | 0.588 |
Number of reflections | 259406 | 2967 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 93.9 | |
Redundancy | 4.4 | |
CC(1/2) | 0.998 | 0.569 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 25 mM Tris-HCl pH8.5, 14-16 % PEG 8K, 20 % w/v |