7PRV

The glucocorticoid receptor in complex with fluticasone furoate, a PGC1a coactivator fragment and sgk 23bp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-07
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.96545
Spacegroup name	P 21 21 21
Unit cell lengths	79.674, 119.725, 135.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.680 - 2.700
R-factor	0.2161
Rwork	0.214
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g9o 4p6w
RMSD bond length	0.008
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	autoPROC (1.1.7)
Phasing software	MOLREP
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.684	3.005
High resolution limit [Å]	2.700	2.700
Rmerge	0.067	1.035
Rpim	0.029	0.425
Number of reflections	24963	1248
<I/σ(I)>	16.3	1.6
Completeness [%]	93.9	63.5
Redundancy	6.5	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	8 % PEG3350, 0.3 M 1,6-hexanediol, 0.1 M guanidine hydrochloride, 8 % 2,2,2-trifluoroethanol, 0.1 M Bis Tris Propane pH 6.5