7PLK
Crystal structure bovine Hsc70(aa1-554)E213A/D214A in complex with nicotinic-acid-derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.976300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.078, 48.799, 86.476 |
Unit cell angles | 90.00, 99.55, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.488 |
R-factor | 0.229670514692 |
Rwork | 0.223 |
R-free | 0.28621 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fl9 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.238 |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.577 |
High resolution limit [Å] | 2.488 | 2.488 |
Rmerge | 0.185 | 1.643 |
Rmeas | 0.201 | |
Rpim | 0.077 | 0.678 |
Number of reflections | 19088 | 1741 |
<I/σ(I)> | 4.8 | 1.43 |
Completeness [%] | 98.2 | |
Redundancy | 6.6 | |
CC(1/2) | 0.993 | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291 | PEG 8000, TMAO, potassium chloride, HEPES-NaOH, glycerol |