7PLI

DEAD-box helicase DbpA bound to single stranded RNA and ADP/BeF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9766
Spacegroup name	P 1
Unit cell lengths	58.643, 121.599, 122.417
Unit cell angles	97.16, 92.43, 103.44

Refinement procedure

Resolution	56.880 - 2.500
R-factor	0.2149
Rwork	0.213
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2db3 3moj
RMSD bond length	0.004
RMSD bond angle	0.834
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874+SVN)
Refinement software	PHENIX (1.18.2_3874+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.880	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	108844	10869
<I/σ(I)>	7.25	1.75
Completeness [%]	96.6	96.52
Redundancy	3.6	3.6
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	20 % (v/v) PEG 3350, 400 mM KSCN, 100 mM Tris, pH 8.0