7PEE

Crystal structure of extracellular part of human Trop2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 43 2 2
Unit cell lengths	145.080, 145.080, 217.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.370 - 2.810
R-factor	0.2408
Rwork	0.240
R-free	0.26330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mzv
RMSD bond length	0.007
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	2.980
High resolution limit [Å]	2.810	2.810
Number of reflections	57322	9084
<I/σ(I)>	15.14	1.85
Completeness [%]	99.6	99.4
Redundancy	6.6
CC(1/2)	0.998	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	294	1.6 M ammonium sulfate, 0.5 M NaCl, 0.0133 M EDTA, 0.1 M MES pH 5.8