7PE9
cryo-EM structure of DEPTOR bound to human mTOR complex 2, DEPt-bound subset local refinement
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.7 |
| Software | cryoSPARC,RELION,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
