7PE8
cryo-EM structure of DEPTOR bound to human mTOR complex 2, focussed on one protomer
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.2 |
Software | cryoSPARC,RELION |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |