7PC8
The PDZ domain of SNTG1 complexed with the phosphomimetic mutant PDZ-binding motif of RSK1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-14 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 226.580, 59.820, 125.210 |
Unit cell angles | 90.00, 117.84, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.334 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmeas | 0.198 | 1.660 |
Number of reflections | 51681 | 3761 |
<I/σ(I)> | 9.56 | 1.26 |
Completeness [%] | 99.5 | |
Redundancy | 6.83 | |
CC(1/2) | 0.995 | 0.532 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Lithium sulfate, 0.05 M Magnesium chloride hexahydrate ,0.1 M HEPES 7.8, 20 % v/v PEG Smear High |