7PC8

The PDZ domain of SNTG1 complexed with the phosphomimetic mutant PDZ-binding motif of RSK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	226.580, 59.820, 125.210
Unit cell angles	90.00, 117.84, 90.00

Refinement procedure

Resolution	48.334 - 2.500
R-factor	0.2037
Rwork	0.202
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2pdz
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.334	2.560
High resolution limit [Å]	2.500	2.500
Rmeas	0.198	1.660
Number of reflections	51681	3761
<I/σ(I)>	9.56	1.26
Completeness [%]	99.5
Redundancy	6.83
CC(1/2)	0.995	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M Lithium sulfate, 0.05 M Magnesium chloride hexahydrate ,0.1 M HEPES 7.8, 20 % v/v PEG Smear High