7PC5

The third PDZ domain of PDZD7 complexed with the PDZ-binding motif of EXOC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-14
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	54.110, 60.350, 181.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.748 - 1.700
R-factor	0.1672
Rwork	0.166
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 3lny
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.748	1.740
High resolution limit [Å]	1.700	1.700
Rmeas	0.100	1.561
Number of reflections	64942	4670
<I/σ(I)>	16.64	1.83
Completeness [%]	97.6
Redundancy	13.64
CC(1/2)	0.999	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M ammonium sulfate, 15% PEG 4000, 0.1M TRIS pH8.5