7PC3

The second PDZ domain of DLG1 complexed with the PDZ-binding motif of HTLV1-TAX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	55.670, 61.320, 143.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.553 - 1.950
R-factor	0.1726
Rwork	0.171
R-free	0.20110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d 2x7z
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.553	2.000
High resolution limit [Å]	1.950	1.950
Rmeas	0.178	1.605
Number of reflections	36311	2616
<I/σ(I)>	12.58	1.79
Completeness [%]	99.2	98.2
Redundancy	13.37
CC(1/2)	0.998	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Lithium sulfate, 0.1 M bis-Tris pH 5.5, 25% w/v PEG 3350