7PB0
Structure of the human heterotetrameric cis-prenyltransferase complex in complex with magnesium, GGsPP and IsPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 185.119, 185.119, 113.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.198 - 2.301 |
| R-factor | 0.1934 |
| Rwork | 0.191 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z1n |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.691 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.200 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.140 | 1.112 |
| Number of reflections | 32739 | 5106 |
| <I/σ(I)> | 8.23 | 1.4 |
| Completeness [%] | 99.3 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.995 | 0.547 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1 M KCl, 0.1 M MES, 36% w/v PEG 400 |
