7PA8

JC polyomavirus VP1 in complex with Fab 27C2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99
Spacegroup name	P 1 21 1
Unit cell lengths	137.070, 110.440, 158.210
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	49.007 - 3.150
Rwork	0.212
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nxg 5bvj
RMSD bond length	0.009
RMSD bond angle	1.604
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.007	3.340
High resolution limit [Å]	3.150	3.150
Rmeas	0.320	1.050
Number of reflections	81593	12843
<I/σ(I)>	9.49	1.6
Completeness [%]	99.6
Redundancy	7.03
CC(1/2)	0.980	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.1 M Ammonium citrate tribasic pH 7.0, 12% (w/v) PEG 3350