7PA6

JC polyomavirus VP1 in complex with scFv 27C11

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-04-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.99
Spacegroup name	P 1
Unit cell lengths	96.090, 96.110, 190.410
Unit cell angles	100.91, 93.01, 113.83

Refinement procedure

Resolution	47.809 - 1.900
Rwork	0.183
R-free	0.21640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nxg 4kq3
RMSD bond length	0.010
RMSD bond angle	1.604
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.500	2.010
High resolution limit [Å]	1.900	1.900
Rmeas	0.100	1.190
Number of reflections	465782	252959
<I/σ(I)>	9.27	1.15
Completeness [%]	89.9
Redundancy	3.83
CC(1/2)	0.990	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	100 mM potassium phosphate monohydrate / potassium phosphate dihydrate pH 6.0, 10% (w/v) PEG 8000, 686 mM NaCl