7P9O

Structure of E.coli RlmJ in complex with a SAM analogue (CA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9786
Spacegroup name	C 1 2 1
Unit cell lengths	79.990, 38.890, 90.650
Unit cell angles	90.00, 105.62, 90.00

Refinement procedure

Resolution	43.651 - 2.095
R-factor	0.2434
Rwork	0.240
R-free	0.30560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qdx
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.651	2.160
High resolution limit [Å]	2.095	2.100
Rmerge	0.083	0.442
Rmeas	0.091	0.479
Rpim	0.035	0.184
Number of reflections	15974	1224
<I/σ(I)>	12.9
Completeness [%]	99.2	92.6
Redundancy	6.7	6.5
CC(1/2)	0.998	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.1 M Bis-Tris pH 6.5 28% (w/v) PEG MME 2000