7P9O
Structure of E.coli RlmJ in complex with a SAM analogue (CA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9786 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.990, 38.890, 90.650 |
Unit cell angles | 90.00, 105.62, 90.00 |
Refinement procedure
Resolution | 43.651 - 2.095 |
R-factor | 0.2434 |
Rwork | 0.240 |
R-free | 0.30560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qdx |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.651 | 2.160 |
High resolution limit [Å] | 2.095 | 2.100 |
Rmerge | 0.083 | 0.442 |
Rmeas | 0.091 | 0.479 |
Rpim | 0.035 | 0.184 |
Number of reflections | 15974 | 1224 |
<I/σ(I)> | 12.9 | |
Completeness [%] | 99.2 | 92.6 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5 28% (w/v) PEG MME 2000 |