7P9I

Structure of E.coli RlmJ in complex with an RNA conjugate (GAA-SAM)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	P 1 21 1
Unit cell lengths	62.480, 57.590, 79.440
Unit cell angles	90.00, 98.14, 90.00

Refinement procedure

Resolution	42.148 - 1.594
R-factor	0.1897
Rwork	0.188
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qdx
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.460	46.460	1.640
High resolution limit [Å]	1.590	7.130	1.590
Rmerge	0.085	0.025	1.418
Rmeas	0.092	0.027	1.541
Rpim	0.035	0.011	0.593
Total number of observations	507892	5497	32747
Number of reflections	74189	884	5089
<I/σ(I)>	14.5	43.8	1.3
Completeness [%]	99.4	99.4	92.3
Redundancy	6.8	6.2	6.4
CC(1/2)	0.999	1.000	0.534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.1 M BIS-TRIS pH 6.5, 20% w/v PEG MME 5000