7P99
Structure of human USPL1 in complex with SUMO2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.709, 69.884, 53.643 |
Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
Resolution | 41.040 - 1.800 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.19740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ALPHAFOLD |
Data reduction software | AutoProcess |
Data scaling software | AutoProcess |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.041 | 1.830 |
High resolution limit [Å] | 1.799 | 1.799 |
Number of reflections | 33606 | 1664 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 96.5 | |
Redundancy | 2.5 | |
CC(1/2) | 0.993 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 311 | 0.2M Potassium thiocyanate, 0.1M Sodium Acetate pH 5.0, 8% PEG20000, 8% PEG500MME |