7P7G
Crystal structure of phosphorylated pT220 Casein Kinase I delta (CK1d), conformation 2 and 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00009 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.542, 64.254, 163.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.830 - 1.700 |
R-factor | 0.1726 |
Rwork | 0.171 |
R-free | 0.20790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ru7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.484 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.830 | 44.830 | 1.740 |
High resolution limit [Å] | 1.700 | 7.970 | 1.700 |
Rmerge | 0.095 | 0.038 | 0.978 |
Rmeas | 0.104 | 0.041 | 1.084 |
Rpim | 0.031 | 0.013 | 0.325 |
Number of reflections | 63264 | 723 | 4246 |
<I/σ(I)> | 16 | 2.4 | |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 10.9 | 9.4 | 10.7 |
CC(1/2) | 0.999 | 0.999 | 0.801 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 12% PEG 3350 and 0.1 M ammonium citrate |