7P7F
Crystal structure of phosphorylated pT220 Casein Kinase I delta (CK1d), conformation 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-06 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 1 |
Unit cell lengths | 49.061, 81.813, 89.088 |
Unit cell angles | 90.27, 105.95, 93.60 |
Refinement procedure
Resolution | 47.090 - 1.960 |
R-factor | 0.1896 |
Rwork | 0.188 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ru7 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.412 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.090 | 47.090 | 2.030 |
High resolution limit [Å] | 1.960 | 7.590 | 1.960 |
Rmerge | 0.048 | 0.032 | 0.413 |
Rmeas | 0.067 | 0.037 | 0.597 |
Rpim | 0.034 | 0.019 | 0.304 |
Number of reflections | 88717 | 1633 | 8568 |
<I/σ(I)> | 12.2 | 2 | |
Completeness [%] | 93.0 | 99.4 | 91.7 |
Redundancy | 3.8 | 3.9 | 3.8 |
CC(1/2) | 0.998 | 0.996 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 277.15 | 20% PEG 3350, 0.1 M sodium sulfate, and 0.1 M citrate, pH 5.9 |