7P71

The PDZ domain of MAGI1_2 complexed with the PDZ-binding motif of HPV35-E6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	192.130, 61.030, 98.990
Unit cell angles	90.00, 97.33, 90.00

Refinement procedure

Resolution	47.640 - 2.600
R-factor	0.2433
Rwork	0.241
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n7d
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.670
High resolution limit [Å]	2.600	2.600
Rmeas	0.050	1.110
Number of reflections	33417	2471
<I/σ(I)>	19.06	1.69
Completeness [%]	94.4	94.5
Redundancy	4.5
CC(1/2)	1.000	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20-25% polyethylene glycol 3000, 100 mM sodium citrate buffered at pH 5.5 and 100 mM trisodium-citrate