7P71
The PDZ domain of MAGI1_2 complexed with the PDZ-binding motif of HPV35-E6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.130, 61.030, 98.990 |
Unit cell angles | 90.00, 97.33, 90.00 |
Refinement procedure
Resolution | 47.640 - 2.600 |
R-factor | 0.2433 |
Rwork | 0.241 |
R-free | 0.28110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n7d |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmeas | 0.050 | 1.110 |
Number of reflections | 33417 | 2471 |
<I/σ(I)> | 19.06 | 1.69 |
Completeness [%] | 94.4 | 94.5 |
Redundancy | 4.5 | |
CC(1/2) | 1.000 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% polyethylene glycol 3000, 100 mM sodium citrate buffered at pH 5.5 and 100 mM trisodium-citrate |