7P5B

Variant Surface Glycoprotein 3 (VSG3, MiTat1.3, VSG224) mutant (serine 319 to alanine), single O-linked glycosylated at Ser317

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	I 21 3
Unit cell lengths	129.007, 129.007, 129.007
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 1.130
R-factor	0.1766
Rwork	0.175
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6elc
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	1.170
High resolution limit [Å]	1.130	1.130
Rmerge	0.078	6.968
Rmeas	0.079	7.094
Rpim	0.013	1.324
Number of reflections	132433	8709
<I/σ(I)>	23.97	0.42
Completeness [%]	96.6	66.13
Redundancy	39	28.4
CC(1/2)	1.000	0.169

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 300mM NaCl, 100mM HEPES, pH 7.5