7P57
VSG2 mutant structure lacking the calcium binding pocket
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-27 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 96.015, 96.015, 108.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.010 - 1.961 |
R-factor | 0.162 |
Rwork | 0.160 |
R-free | 0.19350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vsg |
Data reduction software | xia2 |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.010 | 2.031 |
High resolution limit [Å] | 1.961 | 1.961 |
Rmerge | 0.098 | 0.893 |
Rmeas | 0.101 | 1.023 |
Rpim | 0.021 | 0.475 |
Number of reflections | 36675 | 3341 |
<I/σ(I)> | 20.27 | 0.84 |
Completeness [%] | 99.2 | 92.39 |
Redundancy | 19.7 | 3.6 |
CC(1/2) | 0.999 | 0.557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1M Tris pH 8.0, 39% PEG400 |