7P3I
Crystal structure of human CD40/TNFRSF5 in complex with the anti-CD40 DARPin protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-08 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.999812 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 193.670, 59.564, 81.842 |
Unit cell angles | 90.00, 106.99, 90.00 |
Refinement procedure
Resolution | 92.610 - 2.290 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | none |
RMSD bond length | 0.014 |
RMSD bond angle | 1.587 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 92.610 | 2.326 |
High resolution limit [Å] | 2.286 | 2.286 |
Rmerge | 0.078 | 1.149 |
Number of reflections | 39800 | 8538 |
<I/σ(I)> | 10.1 | 1.3 |
Completeness [%] | 97.8 | 97.3 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 29.9999 %w/v PEG 4K, 0.24 M LiSO4, 0.0999 M TRIS, 0.35 M NaBr |