7P2B
Crystal structure of human gelsolin amyloid mutant A551P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 105 |
Detector technology | PIXEL |
Collection date | 2020-02-10 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 170.070, 170.070, 152.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 3.000 |
R-factor | 0.2154 |
Rwork | 0.213 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ffn |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.910 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 44997 | 3291 |
<I/σ(I)> | 11.53 | |
Completeness [%] | 100.0 | |
Redundancy | 27.3 | |
CC(1/2) | 1.000 | 0.580 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15% glycerol, 2.0 M ammonium sulfate, 0.1M tris HCl, pH8.5 |