7P1I
Cryo EM structure of bison NHA2 in detergent and N-terminal extension helix
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.15 |
| Software | RELION,EPU,CTFFIND |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
