7OZY

FGFR2 kinase domain (residues 461-763) in complex with 38.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-29
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.966
Spacegroup name	P 43 21 2
Unit cell lengths	113.839, 113.839, 117.408
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.500 - 2.280
Rwork	0.240
R-free	0.29590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pvf
RMSD bond length	0.006
RMSD bond angle	1.430
Data reduction software	DIALS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.540	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.141	2.588
Rmeas	0.152	2.777
Rpim	0.056	1.389
Number of reflections	35802	2594
<I/σ(I)>	6.6
Completeness [%]	99.9
Redundancy	7.1
CC(1/2)	0.989	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	291	25% w/v PEG 3350, 0.1 M ammonium sulfate, 0.1 M HEPES