7OZ5
Crystal structure of HIV-1 reverse transcriptase with a double stranded DNA in complex with fragment 166 at the transient P-pocket.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91589 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 310.747, 62.063, 169.292 |
| Unit cell angles | 90.00, 104.93, 90.00 |
Refinement procedure
| Resolution | 150.130 - 3.370 |
| R-factor | 0.2292 |
| Rwork | 0.227 |
| R-free | 0.26460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amo |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.130 | 3.430 |
| High resolution limit [Å] | 3.370 | 3.372 |
| Rmerge | 0.329 | 2.882 |
| Rmeas | 0.362 | 3.168 |
| Rpim | 0.150 | 1.296 |
| Number of reflections | 44790 | 2220 |
| <I/σ(I)> | 3.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.987 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 11-12% v/v PEG Smear Broad, 10% w/v sucrose, 50 mM PIPES-NaOH pH 6.5, 0.1 M (NH4)2SO4, 5 mM MgCl2, 5 mM CdCl2 |
