7OZ2
Crystal structure of HIV-1 reverse transcriptase with a double stranded DNA showing a transient P-pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 310.638, 62.070, 168.239 |
| Unit cell angles | 90.00, 104.51, 90.00 |
Refinement procedure
| Resolution | 81.440 - 2.850 |
| R-factor | 0.219 |
| Rwork | 0.217 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6amo |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.440 | 2.910 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.234 | 1.413 |
| Number of reflections | 73287 | 6000 |
| <I/σ(I)> | 5.9 | 1.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 11-12% v/v PEG Smear Broad, 10% w/v sucrose, 50 mM PIPES-NaOH pH 6.5, 0.1 M (NH4)2SO4, 5 mM MgCl2, 5 mM CdCl2 |
